FastQCFastQC Report
Fri 14 Nov 2014
Hyb_T10_GATCAG_L004_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHyb_T10_GATCAG_L004_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10598840
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTG849180.801200886134709No Hit
AATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAG554450.5231232851897No Hit
GTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAG542690.5120277313366369No Hit
GTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTA459550.43358518479380764No Hit
CTTGTCAGTGACACCGTACTTCTTAGCGACAGTGGCAGTGTCACCACCACC432180.40776160410007134No Hit
GGCAGAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAA431760.4073653343196048No Hit
CTTCAATGGAACACCAGCGTGGTCGTGTTCCAAATCTTGCAAACAAATTTC417550.3939582067471535No Hit
ACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGA412480.3891746643972359No Hit
CTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGGAA379880.3584165814372139No Hit
GACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTG376460.3551898132248435No Hit
ATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTATTA320310.30221231757437605No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC262660.2478195727079567TruSeq Adapter, Index 9 (100% over 51bp)
CGGGCGGTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTT255750.24129999131980484No Hit
CTCCCCCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTC248730.23467662498914973No Hit
CGCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTC242570.2288646682089738No Hit
GGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTT241520.22787399375780748No Hit
GGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGT237030.22363768110472468No Hit
CCCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAA223740.21109857305139051No Hit
GCGCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCC219900.20747553505855357No Hit
CAACAGCGTCTTCGGTGTAACCCAAAACACCCTTCAACTTACCTTCAGCGG211980.2000030191983274No Hit
GTAGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATT210020.1981537602228168No Hit
AGCAGCTGGAGCACCGGTAATGATAACCTTCTTGTTTTCAGAAATTAATTT208050.1962950662525333No Hit
GCCTGCTGCCTTCCTTGGATGTGGTAGCCGTTTCTCAGGCTCCCTCTCCGG207240.19553083167591925No Hit
CTTCACCAGCGTATCTACCGTGAGTGGAGTCGTACTTGAACATGTAAGCAG193740.18279358873235185No Hit
CTAGAAACCAACAAAATAGAACCAAACGTCCTATTCTATTATTCCATGCTA188370.1777269965392439No Hit
CCGTTACCCGTTGAAACCATGGTAGGCCACTATCCTACCATCGAAAGTTGA172800.16303670967766284No Hit
GCCTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAA165400.1560548135456333No Hit
CAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTC163960.15469617429831944No Hit
CTCGTTGAAGAGCAATAATTACAATGCTCTATCCCCAGCACGACGGAGTTT163070.15385645976352127No Hit
ATCAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAGC160130.15108257130025549No Hit
CCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAATAG159930.15089387140479524No Hit
GTGTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTT145760.13752448381143598No Hit
CAGTTTCAGAACAGACGTGGTCTTCACAAGAAGTGATGGTGACCAAAGTGG145430.13721312898392654No Hit
CAACGCAAGCTGATGACTTGCGCTTACTAGGAATTCCTCGTTGAAGAGCAA143200.13510912514954468No Hit
AGCAATGTCAGTGATCATAGCAGTGGTTTCAGAAATGTTGGCAGACATTCT143110.13502421019658756No Hit
CTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCAGAA140190.1322691917228678No Hit
GCCCCCGACCGTCCCTATTAATCATTACGATGGTCCTAGAAACCAACAAAA132550.12506085571628592No Hit
ATCGGTTTCAGGCATTTCCTTCAAACCTATGATGATTCTGTCACGTGGAGC128810.12153216767117911No Hit
GCCTCAAACTTCCATCGGCTTGAAACCGATAGTCCCTCTAAGAAGTGGATA127820.12059810318865083No Hit
GCCCTTCCGTCAATTCCTTTAAGTTTCAGCCTTGCGACCATACTCCCCCCA122580.11565416592759209No Hit
GAAATTTGAATGAACCATCGCCAGCACAAGGCCATGCGATTCGAAAAGTTA119930.11315389231274366No Hit
GTGTACAAAGGGCAGGGACGTAATCAACGCAAGCTGATGACTTGCGCTTAC118570.1118707330236139No Hit
CTGCTTTGAACACTCTAATTTTTTCAAAGTAAAAGTCCTGGTTCGCCAAGA114280.10782312026599139No Hit
CCAGAACCCAAAGACTTTGATTTCTCGTAAGGTGCCGAGTGGGTCATTAAA111920.10559646149956033No Hit
AGCGCGCGTGCGGCCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCC110770.10451143710066384No Hit
CCCAGAACGTCTAAGGGCATCACAGACCTGTTATTGCCTCAAACTTCCATC108790.10264330813560729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCACACG34550.036.9912411
TCACGTG18350.036.66919741
GAACTCC35300.036.45528821
ACACGTC35350.036.34505513
CACACGT35500.036.00133512
GTCACGA34900.035.90633429
CGCGCGC2700.035.843461
CACGTGG18900.035.60260842
ACTCCAG36250.035.3137223
GTATGCC34750.035.03635845
GGTCGTG62000.034.944121
CGTGGAG19700.034.84322444
CGTCTGA37500.034.56128316
GTCGTGT63750.034.0907222
CGTATGC35650.034.02623744
GTGACAC63900.033.9754878
ACGTCTG38450.033.824415
GCGTGGT64250.033.76000617
AGTCACG37700.033.47825228
AGCGTGG66750.033.20348416
Produced by FastQC (version 0.11.2)