FastQCFastQC Report
Fri 14 Nov 2014
lane8_Undetermined_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane8_Undetermined_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4982339
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT269650.043.56373612
GCACACG271100.043.46346711
CACGTCT269550.043.162714
ACGTCTG270050.043.14681215
ACACGTC270000.043.13241213
CGTCTGA272050.043.0694316
GATCGGA290400.042.3261571
GAACTCC271050.042.30557321
AGAGCAC283800.042.239098
CTGAACT275400.042.0609619
CGGAAGA291200.041.9962124
TGAACTC276150.041.9801320
AGCACAC282100.041.9028410
GAGCACA285350.041.6398359
ATCGGAA295250.041.6398242
CCAGTCA229000.041.54439526
AACTCCA267850.041.53531322
ACTCCAG256250.041.51521323
CAGTCAC210300.041.39824727
TCTGAAC281500.041.34062218
Produced by FastQC (version 0.11.2)