FastQCFastQC Report
Fri 14 Nov 2014
lane7_Undetermined_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane7_Undetermined_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4819665
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC105100.2180649484974578TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT222350.043.43598612
GCACACG225050.043.07470311
ACGTCTG222850.042.9551815
CACGTCT224400.042.75866314
CGTCTGA223150.042.75372716
ACACGTC227400.042.23410813
CTGAACT224750.041.93663419
GATCGGA240050.041.754551
CGGAAGA241250.041.4019554
AGAGCAC236950.041.3941888
GTCTGAA230300.041.3369617
TCTGAAC230600.041.04572318
ATCGGAA244250.040.92272
GAGCACA240500.040.701719
GAACTCC228400.040.57753421
AACTCCA223650.040.09253722
CAGTCAC168450.040.0365627
AGCACAC243350.039.97871410
TCCAGTC199900.039.32825525
AAGAGCA252500.039.182747
Produced by FastQC (version 0.11.2)