FastQCFastQC Report
Fri 14 Nov 2014
MK222_GTGGCC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK222_GTGGCC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4078241
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC391670.9603895404906184TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGCC49200.040.55985633
CACACGT57750.038.95751612
GTATGCC50850.038.86224445
GCACACG57900.038.7400211
ACGTCTG58300.038.3198615
CTGAACT58300.038.28080419
CGTCTGA58500.038.03502316
GTGGCCA52450.037.91793434
CACGTCT59500.037.58483514
TCTGAAC60000.037.3461618
GCCATCT54100.036.60186437
GTCACGT57200.036.58334429
TCACGTG56600.036.09656530
AGCACAC62850.035.83164210
GGCCATC55150.035.8216436
CGTATGC55250.035.5229144
CAGTCAC61450.035.15165727
CTCGTAT56000.035.04500642
TCGTATG56650.034.8436843
CCAGTCA63250.034.57818626
Produced by FastQC (version 0.11.2)