FastQCFastQC Report
Fri 14 Nov 2014
MK221_CAGATC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK221_CAGATC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3081337
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATG2200.026.58948929
CTTACAC3000.024.7470728
ACCTATA3150.024.28284313
ACTTACA3300.023.1790777
CTATACG3200.022.4973415
AGTCACC5150.022.28036528
CACCTAT3450.022.1712912
AACTTAC3550.021.5467476
GCTATGG2950.021.35479430
ATACGCC3500.021.21177717
TATACGC3400.021.17396516
ACGTCTG8750.020.31188215
GCCGGTT5550.020.2757681
GTCGCTA2900.020.17133927
CAGGCTA1351.9590516E-920.007742
CACACGT8650.019.76644712
GCCGGTA1950.019.6207071
GCACACG9050.019.38997311
CCTGAGG6950.019.0984616
CACGTCT9350.019.00844614
Produced by FastQC (version 0.11.2)