FastQCFastQC Report
Fri 14 Nov 2014
MK220_ACTTGA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK220_ACTTGA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4791731
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC120720.2519340088164381TruSeq Adapter, Index 8 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC17800.034.7627929
CACACGT22700.031.71584312
GCACACG23250.031.54617911
CACGTCT23950.030.06052814
GTATGCC21700.029.76778445
CGTCTGA24650.029.57165716
ACGTCTG24550.029.233915
ACACTTG22200.027.97393232
CGTATGC23700.027.44567144
CTCGTAT23950.027.2525942
AGCACAC28000.026.3536510
GTCTGAA28150.025.9748217
CTGAACT28200.025.84736619
AGTCACA23950.025.4604328
TCACACT26450.025.26578130
CACACTT27450.024.59126731
TCGTATG26400.024.5534743
TCTGAAC30850.024.06462918
ACACGTC30750.023.63249213
CAGTCAC27800.023.22945827
Produced by FastQC (version 0.11.2)