FastQCFastQC Report
Fri 14 Nov 2014
MK219_ACAGTG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK219_ACAGTG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653545
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC143052.1888316795323965TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACAC17200.042.38213729
GCACACG18150.042.1504111
ACACAGT17100.041.9721132
CACACGT18300.041.92786812
CGTCTGA17900.041.73347516
CACGTCT18050.041.63597514
AGCACAC18500.041.58985510
CACAGTG17300.041.4837133
CACACAG17300.041.35683431
TCACACA17700.041.05778530
GTCTGAA18350.040.9552817
ACACGTC18600.040.888713
ACGTCTG18400.040.8439915
AGTCACA17950.040.73663728
CTCGTAT17650.040.54603642
GTATGCC17900.040.36000445
CTGAACT18600.040.0357619
TCTGAAC18900.039.6383418
CGTATGC18250.039.46265844
CAGTCAC18650.039.44892527
Produced by FastQC (version 0.11.2)