FastQCFastQC Report
Fri 14 Nov 2014
MK218_CGATGT_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK218_CGATGT_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10430471
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC8280257.938519746615469TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC954400.043.68731745
CACGTCT991100.043.42483514
CTCGTAT959100.043.40908442
ACACGTC993650.043.3654713
AGTCACC974400.043.32735428
CACACGT1000300.043.3245812
TCGTATG964550.043.26026543
GAACTCC994150.043.2047921
GATGTAT963150.043.18469635
ACGTCTG997850.043.17843215
CGTATGC964450.043.16906444
TCTCGTA965000.043.15197841
GTATCTC965600.043.1380238
CTCCAGT992600.043.1346224
CGTCTGA998850.043.1284516
GCACACG1005850.043.12578611
ACTCCAG994100.043.09380323
TGTATCT966550.043.08485837
CAGTCAC988450.043.06431227
TCCAGTC992200.043.05986825