FastQCFastQC Report
Fri 14 Nov 2014
MK217_ATCACG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK217_ATCACG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14470808
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC9551506.600529839107809TruSeq Adapter, Index 1 (100% over 51bp)
GGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGA451000.31166193345941706No Hit
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTC252240.17430954788426467No Hit
GCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCTCT223000.1541033506905765No Hit
GCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAA219520.1516985091641047No Hit
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGAT202130.13968121199590236No Hit
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC173920.12018679261033662No Hit
GGCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCTC167530.11577100601431517No Hit
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGGACGCTTGGCTGCCAC164390.11360112026916536No Hit
GTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCG160840.11114790549359788No Hit
CCCGGTTCAAACCAATGCGATAAAAATGGTTTCCGCAACAACGAAGTCGTT153530.10609635619517584No Hit
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAG153030.10575083298734944No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG1140350.043.91243415
GTATGCC1095450.043.8612845
CACACGT1151500.043.8565112
CGTCTGA1145050.043.6968216
GTCTGAA1144250.043.69077317
CACGTCT1147200.043.6894514
GCACACG1160650.043.59605811
CTGAACT1146650.043.44175719
TCTGAAC1150100.043.4033818
GTCACAT1128000.043.2979929
ACATCAC1115750.043.2638532
ACACGTC1169250.042.98484813
CTCGTAT1122100.042.830542
TCACATC1136450.042.76223430
CACATCA1139150.042.70828231
CGTATGC1125900.042.64106444
TCTCGTA1134100.042.4311141
CATCACG1132900.042.4023133
GAGCACA1201450.042.364769
TCGTATG1136350.042.35574343