Basic Statistics
Measure | Value |
---|---|
Filename | MK217_ATCACG_L007_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 14470808 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 50 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC | 955150 | 6.600529839107809 | TruSeq Adapter, Index 1 (100% over 51bp) |
GGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGA | 45100 | 0.31166193345941706 | No Hit |
GTACGATTGAGTGCAAATTAATAGCCTCCCTTGGTGTTTTAAGGGCCGTTC | 25224 | 0.17430954788426467 | No Hit |
GCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCTCT | 22300 | 0.1541033506905765 | No Hit |
GCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGAA | 21952 | 0.1516985091641047 | No Hit |
CAGCTTTGCAACCATACTACCCCCGGAACCGAAAGACTTTCGTTTCCGGAT | 20213 | 0.13968121199590236 | No Hit |
GCCCCAGTCAAACTCCCCGCTTGACACTGTCTTCGAGGAGAGTCACGGTGC | 17392 | 0.12018679261033662 | No Hit |
GGCATCGTTTACGGTCAGAACTAGGGCGGTATCTAATCGCCTTCGAACCTC | 16753 | 0.11577100601431517 | No Hit |
CCGACATCGAAGGATCAAAAAGCAACGTCGCTATGGACGCTTGGCTGCCAC | 16439 | 0.11360112026916536 | No Hit |
GTCGGATTCCCTGAGTCCGTACCGGTTCCAGTACGGTTGTTTAGATGAGCG | 16084 | 0.11114790549359788 | No Hit |
CCCGGTTCAAACCAATGCGATAAAAATGGTTTCCGCAACAACGAAGTCGTT | 15353 | 0.10609635619517584 | No Hit |
CTAAGAAGTTATACGAACCCGAAGATTCGCCAACTATTTAGCAGGCTAGAG | 15303 | 0.10575083298734944 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGTCTG | 114035 | 0.0 | 43.912434 | 15 |
GTATGCC | 109545 | 0.0 | 43.86128 | 45 |
CACACGT | 115150 | 0.0 | 43.85651 | 12 |
CGTCTGA | 114505 | 0.0 | 43.69682 | 16 |
GTCTGAA | 114425 | 0.0 | 43.690773 | 17 |
CACGTCT | 114720 | 0.0 | 43.68945 | 14 |
GCACACG | 116065 | 0.0 | 43.596058 | 11 |
CTGAACT | 114665 | 0.0 | 43.441757 | 19 |
TCTGAAC | 115010 | 0.0 | 43.40338 | 18 |
GTCACAT | 112800 | 0.0 | 43.29799 | 29 |
ACATCAC | 111575 | 0.0 | 43.26385 | 32 |
ACACGTC | 116925 | 0.0 | 42.984848 | 13 |
CTCGTAT | 112210 | 0.0 | 42.8305 | 42 |
TCACATC | 113645 | 0.0 | 42.762234 | 30 |
CACATCA | 113915 | 0.0 | 42.708282 | 31 |
CGTATGC | 112590 | 0.0 | 42.641064 | 44 |
TCTCGTA | 113410 | 0.0 | 42.43111 | 41 |
CATCACG | 113290 | 0.0 | 42.40231 | 33 |
GAGCACA | 120145 | 0.0 | 42.36476 | 9 |
TCGTATG | 113635 | 0.0 | 42.355743 | 43 |