FastQCFastQC Report
Fri 14 Nov 2014
MK216_GTGAAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK216_GTGAAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17536594
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG3509562.0012780132789754TruSeq Adapter, Index 19 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG482300.041.69865415
CACACGT489900.041.5523412
CGTGAAA458050.041.3950533
CGTCTGA488250.041.2043216
ACGTGAA462600.041.1407732
CACGTGA462850.041.05640831
GTCTGAA492300.040.98803717
CAGTCAC486400.040.8032227
GTCACGT473850.040.7918129
TCGTATG448150.040.67268845
TCACGTG470550.040.5662730
GCACACG504500.040.41672511
CACGTCT497350.040.4051714
CTGAACT499600.040.0867319
CTCGTAT461900.040.0028444
GTGAAAC462350.039.91579434
TCTGAAC504150.039.80973418
TGAAACG463900.039.7595435
AAACGAT471700.038.83540737
AGTCACG508000.038.81139428
Produced by FastQC (version 0.11.2)