FastQCFastQC Report
Fri 14 Nov 2014
MK215_GTCCGC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK215_GTCCGC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19350652
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACA552000.042.1318236
CGCACAT558850.041.87405437
ACGTCTG607900.041.7805515
CACACGT613350.041.50834312
CGTCTGA612600.041.4379616
TCGTATG558400.041.42307745
GTCTGAA619500.041.1317417
CAGTCAC615150.040.88857327
GTCACGT600700.040.8271329
TCCGCAC574400.040.79107735
CACGTCT621700.040.7481914
GCACACG625950.040.70155711
CTGAACT621850.040.61078619
TCACGTC601500.040.4885430
CTCGTAT589200.040.42280644
CGTCCGC588700.040.34094233
TCTGAAC628950.040.2237818
TCTCGTA601100.039.76483543
GTCCGCA593700.039.75939634
GCACATC592850.039.6483938
Produced by FastQC (version 0.11.2)