FastQCFastQC Report
Fri 14 Nov 2014
MK214_GTAGAG_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK214_GTAGAG_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17803843
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC148550.038.50135845
TCACGTA151400.038.41583630
ACGTCTG169700.036.9225915
CACACGT174250.036.42328612
CACGTAG161900.035.85489731
CGTATGC159650.035.76807844
CGTCTGA176350.035.5557916
GTCTGAA182900.034.4412217
CAGTCAC179250.034.0664127
GCACACG187750.033.93611511
TCGTATG169000.033.88223343
GTCACGT176800.033.4185929
CACGTCT188600.033.34179314
ACGTAGA171400.033.19701432
CTCGTAT173700.032.85918842
CTGAACT190850.032.84151519
TCTGAAC192900.032.585818
AGTCACG189550.031.78794528
ACACGTC202350.031.3430113
TCTCGTA187250.030.60072941
Produced by FastQC (version 0.11.2)