FastQCFastQC Report
Fri 14 Nov 2014
MK213_CCGTCC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK213_CCGTCC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19371445
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG2508191.2947872499960638TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCCG369250.043.40933630
GTCACCC373650.043.3256929
CACCCGT366600.043.23826231
ACCCGTC366850.043.00528732
CCGTCCC360450.042.6797934
CGTCCCG355450.042.6746335
AGTCACC385900.042.2593828
CCCGTCC367550.042.24096733
ACGTCTG416750.040.7205315
GTCCCGA343250.040.5903336
CACACGT423550.040.2951812
CGTCTGA421750.040.23777416
GTCTGAA428150.039.70399517
CAGTCAC418100.039.53753327
CACGTCT432500.039.2480514
GCACACG437300.039.19281411
CTGAACT434900.038.9892719
TCGTATG337050.038.84506245
TCTGAAC440400.038.63005418
CTCGTAT357000.038.53418744
Produced by FastQC (version 0.11.2)