FastQCFastQC Report
Fri 14 Nov 2014
MK212_ATGTCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK212_ATGTCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20265859
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG5361222.6454442419637876TruSeq Adapter, Index 15 (97% over 40bp)
GGCATAAGCTCTCGTGTTGCCACGAGATAGAGATGCCTCCCGACACCGGGA250510.12361183407029526No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATT239920.11838629687495605TruSeq Adapter, Index 15 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG749350.041.9796715
CACATGT717950.041.91933431
TCACATG724250.041.88089430
GTCACAT727500.041.86389529
CGTCTGA751250.041.8375616
GTCTGAA753150.041.80278417
ATGTCAG715700.041.7484434
CACACGT758700.041.678812
ACATGTC721300.041.55777732
AGTCACA744350.041.3817228
TGTCAGA722250.041.3746835
CATGTCA723150.041.36169433
CTGAACT761550.041.02467319
TCGTATG678750.040.93054645
TCTGAAC765200.040.90249318
CACGTCT767500.040.88139314
CTCGTAT711750.040.8448444
GCACACG775150.040.8320411
CAGTCAC759750.040.76799427
TCTCGTA730500.040.03043443
Produced by FastQC (version 0.11.2)