FastQCFastQC Report
Fri 14 Nov 2014
MK211_AGTTCC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK211_AGTTCC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9381554
Sequences flagged as poor quality0
Sequence length51
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATG7785308.298518560997465TruSeq Adapter, Index 14 (97% over 44bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTATT242750.258752441226688TruSeq Adapter, Index 14 (97% over 44bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT997850.044.1380312
GTCTGAA992600.044.0210117
AGAGCAC1005550.043.99738
CGTCTGA994300.043.94262716
TCGGAAG1005350.043.9392553
TCTGAAC994250.043.9090218
ACACGTC998800.043.86403713
CACGTCT996150.043.85198614
ACGTCTG996850.043.84375815
ATCGGAA1009100.043.8311122
AAGAGCA1011450.043.8118257
AGCACAC1007500.043.77285810
AGTCACA978400.043.77052328
GAGCACA1010550.043.7105949
GATCGGA1012350.043.69761
CACAGTT965200.043.67214231
GTCACAG977400.043.64956329
CTGAACT998650.043.61239619
TCACAGT973300.043.60689530
ACTCCAG995200.043.59629423
Produced by FastQC (version 0.11.2)