FastQCFastQC Report
Fri 14 Nov 2014
MK210_AGTCAA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK210_AGTCAA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16287046
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTCAAATCTCGTATGCC297368218.257957888741764TruSeq Adapter, Index 13 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA3522550.044.5962331
ATCGGAA3520600.044.570262
TCGGAAG3529500.044.3993
CACACGT3512850.044.39744612
GAGCACA3533250.044.3520139
AGAGCAC3540900.044.3335728
CGGAAGA3548550.044.328124
AAGAGCA3548500.044.2772947
GGAAGAG3567950.044.232795
AGCACAC3536800.044.2246210
ACACGTC3507750.044.22211513
CACGTCT3504500.044.1835214
GAAGAGC3560050.044.181676
GCACACG3534650.044.1758211
CGTCTGA3501450.044.15247716
ACGTCTG3503700.044.13645615
GTCTGAA3497800.044.0881217
TCTGAAC3500000.044.00059518
CTGAACT3501650.043.86421619
GAACTCC3529300.043.67801721
Produced by FastQC (version 0.11.2)