FastQCFastQC Report
Fri 14 Nov 2014
MK209_CTTGTA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK209_CTTGTA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10358237
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC10061909.71391173999977TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1192750.044.2562912
GTATGCC1127900.044.25333845
ACGTCTG1187050.044.19586615
GCACACG1196650.044.18349511
CACGTCT1188950.044.10820814
CGTCTGA1191650.044.0365916
AGTCACC1164650.044.0069928
GTCACCT1161650.043.94244429
ACACGTC1196150.043.88408713
GTCTGAA1197300.043.7976617
AGCACAC1208650.043.78391310
GATCGGA1210100.043.7656361
CTGAACT1196450.043.672719
TCTGAAC1202350.043.56318318
ACCTTGT1150350.043.4872232
CAGTCAC1194350.043.45144727
CGGAAGA1227000.043.3701634
CACCTTG1171300.043.3076431
TCACCTT1178650.043.29337330
AGAGCAC1228950.043.2152528
Produced by FastQC (version 0.11.2)