FastQCFastQC Report
Fri 14 Nov 2014
MK208_GGCTAC_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK208_GGCTAC_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12835147
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTACA62200.038.01487434
GCTACAT63250.037.8845835
CGGCTAC64100.037.41439433
CTACATC63400.037.40822236
ACGGCTA66200.036.9772132
GTATGCC68700.036.66055345
CACGGCT70200.035.44852431
CACACGT84250.034.47527312
ACGTCTG84500.034.16027515
TCACGGC75150.033.11359430
CGTCTGA88000.032.75049216
GCACACG89300.032.65163411
TACATCT76700.031.47979737
CACGTCT91950.031.41699614
ACACGTC95200.030.46262713
GTCTGAA95250.030.3747117
CTCGTAT85300.029.41819242
CTGAACT99900.028.98316619
TCTGAAC101700.028.66928318
AGCACAC104050.028.15249610
Produced by FastQC (version 0.11.2)