FastQCFastQC Report
Fri 14 Nov 2014
MK207_TGACCA_L007_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK207_TGACCA_L007_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2804948
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1572195.605059345128679TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT199200.043.99033712
GCACACG199450.043.9239211
CACGTCT198050.043.8141114
GATCGGA201250.043.7374731
ACACGTC198950.043.7289813
AGCACAC200900.043.6845110
CGTCTGA198600.043.53417216
AGAGCAC203550.043.502628
TCGGAAG202350.043.4960983
ACGTCTG199450.043.46144515
GAGCACA204400.043.2446679
ATCGGAA203850.043.1886142
TCTGAAC200400.043.13115318
GTATGCC189350.043.1136745
AGTCACT197000.043.0480328
CTCGTAT190000.043.046842
GTCTGAA201900.043.03433617
CTGAACT200350.043.02962519
CAGTCAC199650.042.89363527
TCCAGTC200350.042.85607525
Produced by FastQC (version 0.11.2)