FastQCFastQC Report
Fri 14 Nov 2014
MK206_TTAGGC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK206_TTAGGC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21523506
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTAGGCATCTCGTATGCC2000710.9295465153307272TruSeq Adapter, Index 3 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTAGG222600.043.61466632
TCACTTA225450.043.39446630
CACTTAG224550.043.31788631
CTTAGGC222750.042.74374833
GTATGCC242950.040.79644845
TTAGGCA233400.040.72531534
AGTCACT248900.039.75808328
ACGTCTG265100.039.21750615
CACACGT270850.038.38493712
CGTCTGA273800.038.34051516
GTCACTT261000.037.9752429
CTCGTAT264000.037.53406542
CACGTCT275750.037.49071514
GCACACG280000.037.19485511
CGTATGC266700.037.16355544
AGGCATC259400.037.08269536
GTCTGAA286800.036.69667417
CAGTCAC278500.036.6230627
TCGTATG274700.036.1631843
CTGAACT296150.035.53718619
Produced by FastQC (version 0.11.2)