FastQCFastQC Report
Fri 14 Nov 2014
MK205_CGATGT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK205_CGATGT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11284071
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC6250215.538967275197045TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT741650.043.95549412
GCACACG746750.043.68843511
AGCACAC750000.043.53134510
GATCGGA770100.043.43051
ACACGTC746900.043.4145913
TCGGAAG769150.043.392253
CGTCTGA756400.043.3747716
CACGTCT747200.043.3489814
AGAGCAC762450.043.2865458
ACGTCTG754600.043.15345415
AGTCACC734300.043.1356328
GTATGCC726450.042.94147545
TCTGAAC761800.042.9218418
GTCTGAA765500.042.9002917
ATCGGAA779900.042.8312
CTCGTAT727250.042.7602242
GAGCACA766950.042.7188849
TATCTCG728000.042.68954539
CAGTCAC754500.042.6873727
GTATCTC729600.042.6100338