Basic Statistics
Measure | Value |
---|---|
Filename | MK205_CGATGT_L008_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 11284071 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 49 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC | 625021 | 5.538967275197045 | TruSeq Adapter, Index 2 (100% over 51bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACACGT | 74165 | 0.0 | 43.955494 | 12 |
GCACACG | 74675 | 0.0 | 43.688435 | 11 |
AGCACAC | 75000 | 0.0 | 43.531345 | 10 |
GATCGGA | 77010 | 0.0 | 43.4305 | 1 |
ACACGTC | 74690 | 0.0 | 43.41459 | 13 |
TCGGAAG | 76915 | 0.0 | 43.39225 | 3 |
CGTCTGA | 75640 | 0.0 | 43.37477 | 16 |
CACGTCT | 74720 | 0.0 | 43.34898 | 14 |
AGAGCAC | 76245 | 0.0 | 43.286545 | 8 |
ACGTCTG | 75460 | 0.0 | 43.153454 | 15 |
AGTCACC | 73430 | 0.0 | 43.13563 | 28 |
GTATGCC | 72645 | 0.0 | 42.941475 | 45 |
TCTGAAC | 76180 | 0.0 | 42.92184 | 18 |
GTCTGAA | 76550 | 0.0 | 42.90029 | 17 |
ATCGGAA | 77990 | 0.0 | 42.831 | 2 |
CTCGTAT | 72725 | 0.0 | 42.76022 | 42 |
GAGCACA | 76695 | 0.0 | 42.718884 | 9 |
TATCTCG | 72800 | 0.0 | 42.689545 | 39 |
CAGTCAC | 75450 | 0.0 | 42.68737 | 27 |
GTATCTC | 72960 | 0.0 | 42.61003 | 38 |