FastQCFastQC Report
Fri 14 Nov 2014
MK204_ATCACG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameMK204_ATCACG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15171247
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC10789727.111953289007819TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1282750.044.29469712
GCACACG1289750.044.1101111
ACACGTC1285050.043.95280513
CACGTCT1285100.043.8863214
AGCACAC1299200.043.87108610
TCGGAAG1324400.043.845383
GATCGGA1327650.043.760871
CGTCTGA1302250.043.750216
ACGTCTG1295800.043.68192715
AGAGCAC1322350.043.5839468
ATCGGAA1334950.043.531252
GTCTGAA1312050.043.4232717
GAGCACA1319500.043.3528639
TCTGAAC1311300.043.345618
AGTCACA1262200.043.33651728
GTATGCC1256600.043.32068645
GTCACAT1257750.043.27337329
CTCGTAT1260700.043.12539342
CAGTCAC1301700.043.0811327
CTGAACT1314700.043.0315819
Produced by FastQC (version 0.11.2)