FastQCFastQC Report
Fri 14 Nov 2014
LW261_GGCTAC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW261_GGCTAC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6347799
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC1639432.582674719221576TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC197350.043.1189745
ACGGCTA196300.042.9688532
CACGGCT197450.042.81142831
GTCACGG201750.042.77999529
AGTCACG203500.042.6442928
TCACGGC201150.042.31476630
GGCTACA195300.042.30048434
CGGCTAC197550.042.21829633
GCACACG212600.042.19211211
CGTCTGA214700.042.18810716
CGTATGC201700.042.0774644
CACGTCT212000.042.0249914
ACGTCTG213200.042.0206115
ACACGTC212250.041.99669313
TCGTATG203600.041.8951543
CACACGT214150.041.85521312
GCTACAT199650.041.8315635
CTCGTAT203150.041.79895442
CAGTCAC212300.041.79868327
CCAGTCA214100.041.69948226