FastQCFastQC Report
Fri 14 Nov 2014
LW260_TAGCTT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW260_TAGCTT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8998757
Sequences flagged as poor quality0
Sequence length51
%GC38

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC3955024.39507367517536TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC457550.043.44334845
CACTAGC451550.043.411431
ACTAGCT451550.043.3793332
TAGCTTA454700.043.11528434
CTAGCTT455500.042.99654433
CGTATGC461700.042.97973644
TCACTAG459950.042.95123330
CGTCTGA476150.042.91200316
ACGTCTG473100.042.8992915
ACACGTC472150.042.86647413
CAGTCAC470600.042.849427
GCACACG475000.042.79873311
CTCGTAT464500.042.7928642
GCTTATC462550.042.7902536
CACACGT475450.042.72983612
CACGTCT473450.042.696514
TCGTATG466550.042.68730543
CCAGTCA476150.042.619326
CTTATCT465950.042.5366737
TATCTCG468650.042.5064239