FastQCFastQC Report
Fri 14 Nov 2014
LW259_GATCAG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW259_GATCAG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7293418
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC4240975.8147908154996735TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC488850.043.79745
ACGTCTG509300.043.53420315
GCACACG512050.043.5200911
ACACGTC509050.043.4937113
CGTCTGA513850.043.44614816
CACACGT512450.043.44221512
CACGTCT509300.043.42376714
AGTCACG499900.043.37373728
GTCACGA492200.043.14251729
CACGATC486450.043.12977631
AGCACAC517900.043.07856410
GAACTCC515550.043.07419621
CCAGTCA512950.043.05986826
CAGTCAC511650.043.0109527
TCCAGTC513750.042.9358825
ACTCCAG515150.042.89860523
GATCGGA532150.042.7787781
CGTATGC500150.042.7399944
TCACGAT495900.042.7344130
ACGATCA489250.042.71561432
Produced by FastQC (version 0.11.2)