FastQCFastQC Report
Fri 14 Nov 2014
LW256_GCCAAT_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW256_GCCAAT_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6324593
Sequences flagged as poor quality0
Sequence length51
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC92272814.589523784376324TruSeq Adapter, Index 6 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT1109600.044.25106412
ACACGTC1101500.044.23536313
CACGTCT1100800.044.19400414
AGCACAC1114750.044.174410
CGTCTGA1114700.044.1434716
GCACACG1114100.044.1268511
ACGTCTG1106850.044.11911815
AGAGCAC1128200.044.116398
TCGGAAG1139300.044.0903473
GAGCACA1121200.044.070779
GATCGGA1142500.044.0239831
TCTGAAC1115650.043.98550418
GTATGCC1076350.043.96148345
CGGAAGA1143300.043.8899044
GAAGAGC1144000.043.8827136
AAGAGCA1142700.043.8814477
ATCGGAA1146450.043.8414652
GTCTGAA1124000.043.8302717
CTGAACT1115550.043.79785519
GGAAGAG1148600.043.783365
Produced by FastQC (version 0.11.2)