FastQCFastQC Report
Fri 14 Nov 2014
LW255_ACAGTG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW255_ACAGTG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5093036
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC438440.8608617728207695TruSeq Adapter, Index 5 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC60850.038.75946844
CACACGT65150.038.29713412
CACAGTG59200.038.156633
GCACACG65450.038.05284511
GTATGCC62600.037.89163645
ACACGTC65650.037.73129713
CTCGTAT62200.037.62840342
CGTCTGA66250.037.62729616
CACGTCT66150.037.48011414
TCGTATG63250.037.32433343
ACGTCTG66750.037.21062515
GTCACAC62300.037.1294229
CCAGTCA65500.036.7239626
AGTGATC63000.036.5382936
ACACAGT61900.036.42278332
GATCTCG64350.036.3337339
CAGTCAC66200.036.1996827
ACTCCAG68650.036.0806723
CACACAG63500.036.0379731
TCCAGTC68350.035.71941825
Produced by FastQC (version 0.11.2)