FastQCFastQC Report
Fri 14 Nov 2014
LW254_TGACCA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW254_TGACCA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6761741
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC1332931.9712822481665595TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC164350.041.84810345
CGTATGC165000.041.62869344
CTCGTAT166700.041.3385642
TCGTATG167300.041.2850843
ACACGTC176400.040.9020213
CTGACCA167450.040.83220333
ACTGACC168250.040.8276832
GCACACG177750.040.73060211
ACGTCTG177750.040.73060215
CGTCTGA178550.040.7110116
CACACGT178150.040.6644112
GTCACTG173750.040.62426429
CCAGTCA177100.040.45295726
TCCAGTC176850.040.39563425
CACGTCT178500.040.37040714
CAGTCAC176600.040.3381527
ACTCCAG177650.040.27139723
TCTCGTA172300.040.0858441
GAACTCC180100.040.02306421
GACCAAT172450.039.896535
Produced by FastQC (version 0.11.2)