FastQCFastQC Report
Fri 14 Nov 2014
LW253_GGTAGC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW253_GGTAGC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4228232
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGCC52134812.330165421386527TruSeq Adapter, Index 22 (97% over 37bp)
GATCGGAAGAGCACACGTCTGTACTCCAGTCACGGTAGCATCTCGTATGCC42830.10129529316272143TruSeq Adapter, Index 11 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACACGT632900.043.8509212
CACGTCT631250.043.7909114
CACGGTA602550.043.7808231
ACGGTAG600700.043.77446732
AGTCACG622350.043.6967428
GTATGCC599500.043.69486245
ACACGTC632850.043.6908613
CGTCTGA635550.043.688816
CTCCAGT634000.043.68619524
GTAGCAT597300.043.68255635
CCAGTCA635450.043.60679226
ACGTCTG636100.043.60202815
CAGTCAC632450.043.5930627
GGTAGCA595950.043.59068734
GTCTGAA637200.043.58626617
AGCACAC639300.043.576310
TCTGAAC636200.043.57241418
GCACACG638300.043.5715911
ACTCCAG636250.043.5700223
AGAGCAC646000.043.5591558
Produced by FastQC (version 0.11.2)