Basic Statistics
Measure | Value |
---|---|
Filename | LW252_GAGTGG_L008_R1_001.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5920014 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 54 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC | 311646 | 5.264278091234244 | TruSeq Adapter, Index 7 (97% over 36bp) |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACGAGTG | 34975 | 0.0 | 43.392048 | 32 |
CACGAGT | 34995 | 0.0 | 43.36265 | 31 |
GTATGCC | 35635 | 0.0 | 43.095074 | 45 |
AGTCACG | 36775 | 0.0 | 43.03819 | 28 |
TCACGAG | 36180 | 0.0 | 42.97479 | 30 |
CACACGT | 37980 | 0.0 | 42.691505 | 12 |
ACTCCAG | 38110 | 0.0 | 42.603474 | 23 |
CTCGTAT | 36040 | 0.0 | 42.60347 | 42 |
CAGTCAC | 37765 | 0.0 | 42.600742 | 27 |
GAGTGGA | 35115 | 0.0 | 42.597805 | 34 |
CGAGTGG | 35500 | 0.0 | 42.561855 | 33 |
CACGTCT | 37890 | 0.0 | 42.5554 | 14 |
CGTCTGA | 38290 | 0.0 | 42.533173 | 16 |
CTCCAGT | 38185 | 0.0 | 42.505352 | 24 |
CCAGTCA | 38170 | 0.0 | 42.467056 | 26 |
TCGTATG | 36275 | 0.0 | 42.45264 | 43 |
ACGTCTG | 38145 | 0.0 | 42.41837 | 15 |
GTCACGA | 36805 | 0.0 | 42.397858 | 29 |
CGTATGC | 36235 | 0.0 | 42.381485 | 44 |
ACACGTC | 38070 | 0.0 | 42.377834 | 13 |