FastQCFastQC Report
Fri 14 Nov 2014
LW252_GAGTGG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW252_GAGTGG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5920014
Sequences flagged as poor quality0
Sequence length51
%GC54

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGCC3116465.264278091234244TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGTG349750.043.39204832
CACGAGT349950.043.3626531
GTATGCC356350.043.09507445
AGTCACG367750.043.0381928
TCACGAG361800.042.9747930
CACACGT379800.042.69150512
ACTCCAG381100.042.60347423
CTCGTAT360400.042.6034742
CAGTCAC377650.042.60074227
GAGTGGA351150.042.59780534
CGAGTGG355000.042.56185533
CACGTCT378900.042.555414
CGTCTGA382900.042.53317316
CTCCAGT381850.042.50535224
CCAGTCA381700.042.46705626
TCGTATG362750.042.4526443
ACGTCTG381450.042.4183715
GTCACGA368050.042.39785829
CGTATGC362350.042.38148544
ACACGTC380700.042.37783413