FastQCFastQC Report
Fri 14 Nov 2014
LW251_CGTACG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW251_CGTACG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11945116
Sequences flagged as poor quality0
Sequence length51
%GC55

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGCC210015517.581704522584797TruSeq Adapter, Index 22 (98% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTACGATCTCGTATGCC356800.29869948521219886TruSeq Adapter, Index 22 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTAC2386300.043.5375332
CACCGTA2429150.043.51774231
AGTCACC2467900.043.48817428
CTCCAGT2547950.043.46362324
CGTACGA2354900.043.45409434
CCAGTCA2556200.043.42429426
GTACGAT2407000.043.42267235
CAGTCAC2543450.043.41461627
ACTCCAG2554250.043.4094723
AACTCCA2554450.043.39884622
CCGTACG2381150.043.3988433
GAACTCC2562200.043.39050321
TCTGAAC2571150.043.38015718
CACACGT2565850.043.3779412
CACGTCT2551400.043.36612714
GTCTGAA2577450.043.345417
CGTCTGA2579350.043.34087416
TGAACTC2573850.043.32084720
TCCAGTC2555150.043.31268725
TACGATC2395600.043.3087336
Produced by FastQC (version 0.11.2)