FastQCFastQC Report
Fri 14 Nov 2014
LW250_GTTTCG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW250_GTTTCG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4416368
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGCC1607983.6409556450005978TruSeq Adapter, Index 21 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC185450.042.92575545
AGTCACG193450.042.80314628
CACGTCT199550.042.4502614
CACACGT200850.042.4443512
GCACACG201350.042.36129811
GTCACGT189950.042.3598929
ACACGTC200100.042.34482213
ACGTCTG201050.042.2902215
CGTATGC188250.042.26337444
CGTCTGA202800.042.24558616
ACTCCAG202750.042.19765523
CAGTCAC202100.042.1290327
TCGTATG190100.042.00599343
CTCGTAT189600.041.97194342
CCAGTCA204650.041.81298426
GAACTCC205350.041.7291121
AGCACAC205600.041.68025610
CTCCAGT205050.041.6412824
TCCAGTC205300.041.57100325
GATCGGA212400.041.331581
Produced by FastQC (version 0.11.2)