FastQCFastQC Report
Fri 14 Nov 2014
LW249_GTGGCC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW249_GTGGCC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4907245
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGCC4388738.943368427702305TruSeq Adapter, Index 20 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC504950.043.6390845
AGTCACG523050.043.4918128
GTCACGT514850.043.38909529
CGTCTGA541050.043.29510516
CACGTCT536000.043.28791414
CACACGT538150.043.26965312
ACACGTC536400.043.24724613
GCACACG539250.043.23562611
ACGTCTG539150.043.23530215
TCACGTG514450.043.2128930
ACTCCAG537700.043.1953223
CAGTCAC534450.043.19141827
GAACTCC539400.043.18803821
CCAGTCA538050.043.10735326
CTCCAGT538750.043.02452524
TCCAGTC538800.042.984725
CGTATGC512150.042.97285544
AGCACAC543650.042.93818710
TGGCCAT506400.042.90991235
CTGAACT543000.042.90170719
Produced by FastQC (version 0.11.2)