FastQCFastQC Report
Fri 14 Nov 2014
LW248_GTGAAA_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW248_GTGAAA_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6203332
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG6158609.9278903660162TruSeq Adapter, Index 19 (97% over 40bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAATGATCTCGTATG76480.12328858103999593TruSeq Adapter, Index 19 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG731650.043.89123545
AGTCACG759200.043.67514428
GTCACGT749950.043.58380529
CACGTGA747950.043.54993431
TCACGTG749250.043.49839830
CAGTCAC771800.043.39650727
ACTCCAG768100.043.3780323
CCAGTCA772950.043.3726926
TCCAGTC771350.043.35181425
ACGTCTG776050.043.33870315
CGTCTGA782200.043.3312616
GAACTCC774250.043.30079321
CACACGT781850.043.2560512
ACACGTC775600.043.22751613
CACGTCT775200.043.21499314
CTCCAGT770900.043.1930324
ACGTGAA753400.043.18836232
GCACACG784150.043.16934211
CTCGTAT745250.043.01778444
TGAACTC786500.043.00394420
Produced by FastQC (version 0.11.2)