FastQCFastQC Report
Fri 14 Nov 2014
LW247_GTCCGC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW247_GTCCGC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7881938
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCACATCTCGTATG99964712.682756448985009TruSeq Adapter, Index 18 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG1188000.044.14739245
ACGTCCG1197350.043.87706832
AGTCACG1241850.043.86207628
GTCACGT1223150.043.79130629
TCACGTC1214550.043.78458830
CACGTCC1202350.043.78159331
CAGTCAC1264700.043.65466327
CCAGTCA1262600.043.63638326
TCCAGTC1259850.043.6173325
CGTCCGC1196800.043.58775733
ACTCCAG1254700.043.58349223
GAACTCC1262200.043.55951721
GTCCGCA1198500.043.54199634
TCCGCAC1200000.043.5350535
CGTCTGA1277300.043.51648716
CTCCAGT1256450.043.48077824
CGCACAT1207400.043.46194537
ACACGTC1264050.043.4041913
CACACGT1269800.043.39368412
GCACACG1269750.043.37590411