FastQCFastQC Report
Fri 14 Nov 2014
LW246_GTAGAG_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW246_GTAGAG_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4925522
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGCC118290.24015728688248678TruSeq Adapter, Index 3 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACG21600.031.04132828
GCACACG25750.031.01656211
ACGTCTG25650.030.69892915
TCACGTA20350.030.62620430
GTCACGT21300.030.31657229
ACACGTC26250.030.25435613
CACGTCT26450.030.11065114
CGTCTGA26350.030.05416116
CACACGT26900.029.85784712
CGTAGAG19850.029.35438333
CGTATGC22550.029.04666144
GTATGCC22900.028.50471745
CAGTCAC24900.027.46958427
CCAGTCA26300.027.4616826
CTCGTAT24000.027.19714542
GAACTCC29500.026.69110321
TCGTATG24350.026.62243343
CACGTAG23600.025.93192131
GATCTCG24200.025.66739539
TCCAGTC29200.025.65896825
Produced by FastQC (version 0.11.2)