FastQCFastQC Report
Fri 14 Nov 2014
LW245_CCGTCC_L008_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameLW245_CCGTCC_L008_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5972442
Sequences flagged as poor quality0
Sequence length51
%GC39

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTATG1387102.3225005784903394TruSeq Adapter, Index 16 (97% over 40bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCCG189250.043.5114935
CCGTCCC189350.043.4967534
GTCCCGA174100.043.33628536
CCCGTCC191200.043.19571733
CACCCGT195900.043.16249531
ACCCGTC192850.043.07473832
GTCACCC201700.043.0703229
TCACCCG199750.043.05148330
GCACACG210850.042.76632711
CGTCTGA212000.042.67194416
ACGTCTG210550.042.64560715
AGTCACC204900.042.5733728
CACGTCT210300.042.53583514
CCCGATC175300.042.33683438
ACACGTC211300.042.31323613
CACACGT213400.042.2025812
CCAGTCA212450.042.1618526
ACTCCAG213300.042.0514923
CCGATCT175250.041.99148639
CAGTCAC211600.041.96968527