FastQCFastQC Report
Fri 14 Nov 2014
lane5_Undetermined_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamelane5_Undetermined_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5240084
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGGC4600.014.65946940
TAGCCTG1000.007396825613.54345127
TGGGGCC2251.3752813E-510.9902243
TTTGTGT41550.010.439582
GTGTTTG46200.010.4102293
CGTGCCG2006.031435E-410.15758930
TGTGTTT47400.010.0520412
TTGTGTT49000.09.9086031
GCCGCGC2302.0720705E-49.77391143
CTTTGTG45050.09.4801141
TGAGGCG3603.267578E-79.36442910
ATTCGGG3603.2793414E-68.741302512
AACTTTG34000.08.4611192
TAACTTT35450.08.4331561
TGTTTGA58550.08.367934
AGGCTAC4752.452889E-78.04462713
GGGGCCC3206.390713E-47.727940644
CTACCCG5005.5631244E-77.642249616
CACGGTA6551.877197E-97.55096545
CTAAGCG5107.611652E-77.492902841
Produced by FastQC (version 0.11.2)