FastQCFastQC Report
Fri 14 Nov 2014
handrnoggin12hpf2_AAGACG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrnoggin12hpf2_AAGACG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26047335
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT240850.011.9108962
GTGTTTG343000.010.1668793
TGTGTTT351400.010.078222
TAACTTT180450.010.0771741
TTGTGTT355450.09.9720941
AACTTTG190200.09.4755162
CTTTGTG324850.09.1032561
TGTTTGA415700.08.50237754
CTACCCG33700.07.67841116
GAGGCTA35400.07.246278312
GCTACCC38300.06.93262115
AGGCTAC36850.06.777971313
GTTTGAG226500.06.674785
GACGGGC45850.06.57523639
ACTTTGT282550.06.48155453
TTTGAGG103750.06.1583756
TGACGGG56450.06.0577978
CTAAGCG42800.05.94136841
TTTGACG122950.05.89200646
CCGCTGA50200.05.736424420
Produced by FastQC (version 0.11.2)