FastQCFastQC Report
Fri 14 Nov 2014
handrnoggin12hpf1_CCTTCA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrnoggin12hpf1_CCTTCA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26120948
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT216450.011.7776932
TAACTTT182900.010.5695881
GTGTTTG309700.010.192493
AACTTTG192400.010.0572982
TTGTGTT315500.010.0505581
TGTGTTT317500.010.0418572
TGTTTGA375350.08.5235474
CTTTGTG310850.08.4488691
GACGGGC41200.07.64502439
TGACGGG50850.07.34446438
CTAGGCC3100.0039614537.258766742
ACTTTGT271200.07.1844253
ACGGGCG42000.07.17800110
CTACCCG30950.06.906584716
GCGGTGT50650.06.797001414
TTGACGG64450.06.6673667
GAGGCTA31050.06.667017512
CGGTGTG52850.06.55663715
GCTACCC33200.06.43859215
TGTGTAC60950.06.348889418
Produced by FastQC (version 0.11.2)