FastQCFastQC Report
Fri 14 Nov 2014
handrlaz12hpf2_TTCAGC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrlaz12hpf2_TTCAGC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26633672
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT215550.011.4192912
GTGTTTG318950.010.6021323
TTGTGTT322850.010.4972711
TGTGTTT324550.010.4749682
TAACTTT184400.09.7385471
TGTTTGA383050.08.8571944
AACTTTG202250.08.810622
CTTTGTG291750.08.7777591
ACTTTGT260850.06.7621073
TTTGAGG104650.06.49244366
GACGGGC40050.06.46023139
TGACGGG48250.06.43458658
ACGGGCG40900.06.215955310
CGGTGTG48600.06.019032515
GTTTGAG272600.05.91743955
CTACCCG23950.05.73099516
TTGACGG63500.05.5270147
GAGGGGC27850.05.49333959
GCGGTGT51000.05.47105514
GAGGCTA26650.05.40383814
Produced by FastQC (version 0.11.2)