FastQCFastQC Report
Fri 14 Nov 2014
handrlaz12hpf1_AAGGGA_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrlaz12hpf1_AAGGGA_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24544920
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT250250.012.964682
TAACTTT190100.011.0685191
CTTTGTG304700.010.7904051
AACTTTG201100.010.438552
GTGTTTG359500.09.5872545
TTGTGTT353700.09.5691371
TGTGTTT363350.09.38688854
ACTTTGT255150.08.1652453
TGTTTGA438100.07.81584176
CTACCCG28400.07.525573318
GAGGCTA27650.07.16144414
GCTACCC32400.07.083557617
AGGCTAC28950.06.76213415
GACGGGC41050.06.74225311
TGACGGG49100.06.369193610
TTTGAGG109950.06.32262756
ACGGGCG42750.06.053057712
CTAAGCG37500.05.880976743
CGGTACT33600.05.69315445
GCGCGGC12705.0931703E-115.670187540
Produced by FastQC (version 0.11.2)