FastQCFastQC Report
Fri 14 Nov 2014
handrdnfgfr12hpf2_CCTCGG_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrdnfgfr12hpf2_CCTCGG_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24468524
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTGTGT199950.011.8265092
GTGTTTG285750.010.8104243
TGTGTTT290650.010.6672922
TTGTGTT298900.010.4051831
TGTTTGA351650.08.8355854
CTTTGTG284100.08.539161
TAACTTT172150.08.536381
AACTTTG184000.08.1683432
TGACGGG47850.07.4757548
GACGGGC40350.07.4716449
ACGGGCG39850.06.887893710
CTACCCG22450.06.715167516
TTGACGG63050.06.3871857
GCGGTGT50500.06.148952514
TGTGTAC61150.06.144121618
ACTTTGT248000.06.01478773
CGGTACT27800.05.99041945
GCTACCC26300.05.9890215
GAGGCTA25300.05.95892312
AGGCTAC25900.05.90775713
Produced by FastQC (version 0.11.2)