FastQCFastQC Report
Fri 14 Nov 2014
handrdnfgfr12hpf1_GGACCC_L005_R1_001.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamehandrdnfgfr12hpf1_GGACCC_L005_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27089652
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT207400.014.7574131
AACTTTG221900.013.9016782
TTTGTGT346500.013.2339052
CTTTGTG448300.010.4267231
TTGTGTT488400.09.8813873
TGTGTTT491650.09.8250924
GTGTTTG498600.09.8141785
ACTTTGT364200.08.5190383
TGTTTGA594450.08.2620046
CTACCCG46950.06.51701518
GACGGGC47100.06.40082179
GAGGCTA47750.06.36159314
GTTTGAG329950.06.17769247
TTTGAGG125550.06.1464178
TGACGGG61600.06.13582758
AGGCTAC49200.06.08263915
CGGTACT55550.06.07669245
GCTACCC51550.06.023474717
ACGGGCG51250.05.882532610
CACGGTA73200.05.718233645
Produced by FastQC (version 0.11.2)