FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l08n01 2-3h gd7 8wg16 rep2 input_150303.34100000007bbc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l08n01 2-3h gd7 8wg16 rep2 input_150303.34100000007bbc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44507282
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT2901410.6518955707068339No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA2511190.5642200303312164No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA2497200.5610767244784798No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2325820.522570666076621No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA2310990.5192386270633197No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1932550.43420984458228656No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1861160.4181697727576355No Hit
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA1813870.4075445451825165No Hit
CAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGAC1681140.3777224589899693No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1660190.3730153640925546No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1646460.3699304756466593No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA1463280.3287731657035359No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1329350.29868146071018226No Hit
ACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGA1231540.2767052816211064No Hit
CAACACAACACAACACAACACAACACAACACAACACAACACAACACAACAC1198700.2693267137723665No Hit
AACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA1123510.2524328490784946No Hit
CACAACACAACACAACACAACACAACACAACACAACACAACACAACACAAC1099870.24712135870260513No Hit
GACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAG1022160.2296612945270394No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC984330.2211615618316122TruSeq Adapter, Index 6 (100% over 51bp)
TGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT910480.20456877146530764No Hit
ACAACACAACACAACACAACACAACACAACACAACACAACACAACACAACA903650.20303419112405022No Hit
GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTG901440.20253764316589812No Hit
TGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT833560.18728620633360626No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTG783790.1761037665701536No Hit
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACAA749060.16830054911014336No Hit
TTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGT600170.13484759640006774No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGC150450.030.26658229
AGTCACG151050.030.21951128
CGTCTGA164750.028.29163716
TATCTCG162000.027.9011239
CACGTCT168400.027.75851614
ACACGTC168400.027.731813
ACGTCTG169350.027.60286115
TCACGCC167800.027.13714430
CTCGTAT167200.027.06032442
TCTGAAC180700.025.76984418
TCGTATG178750.025.4117643
GTATGCC180100.025.37041345
CAGTCAC188150.024.53673427
CGTATGC187450.024.36436544
ATCTCGT190150.023.9481740
CACACGT196700.023.91346412
TCTCGTA190950.023.83600241
ATATCTC191700.023.69569438
GTCTGAA200450.023.38761117
CACGCCA196450.023.33873231
Produced by FastQC (version 0.11.2)