Basic Statistics
Measure | Value |
---|---|
Filename | C55MVACXX l08n01 2-3h gd7 8wg16 rep2_150303.34100000007b86.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 48413980 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC | 1313310 | 2.7126668784512242 | TruSeq Adapter, Index 2 (100% over 51bp) |
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA | 187075 | 0.38640698409839475 | No Hit |
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA | 177899 | 0.36745378091204234 | No Hit |
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA | 154874 | 0.31989520382335845 | No Hit |
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT | 152185 | 0.31434102298550953 | No Hit |
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT | 139225 | 0.2875718955557878 | No Hit |
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC | 135136 | 0.2791259879894196 | No Hit |
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG | 131852 | 0.27234282329195 | No Hit |
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC | 123382 | 0.25484787658440805 | No Hit |
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG | 121665 | 0.2513013803037883 | No Hit |
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT | 116694 | 0.2410336848984529 | No Hit |
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA | 54008 | 0.11155455510990833 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
ACACGTC | 146985 | 0.0 | 43.025425 | 13 |
CACGTCT | 147030 | 0.0 | 42.93733 | 14 |
ACGTCTG | 147335 | 0.0 | 42.810455 | 15 |
CGTCTGA | 147450 | 0.0 | 42.77096 | 16 |
TCGGAAG | 150045 | 0.0 | 42.647217 | 3 |
CGGAAGA | 150450 | 0.0 | 42.469612 | 4 |
GATCGGA | 152025 | 0.0 | 42.31232 | 1 |
CGATGTA | 147050 | 0.0 | 42.22293 | 34 |
CACACGT | 150185 | 0.0 | 42.15212 | 12 |
GTCACCG | 147990 | 0.0 | 42.110096 | 29 |
TATCTCG | 146835 | 0.0 | 42.044544 | 39 |
CCGATGT | 148015 | 0.0 | 42.002377 | 33 |
AGTCACC | 148625 | 0.0 | 41.92987 | 28 |
TCTGAAC | 150680 | 0.0 | 41.79944 | 18 |
ACCGATG | 149185 | 0.0 | 41.76212 | 32 |
TCTCGTA | 147555 | 0.0 | 41.761795 | 41 |
CTCGTAT | 148075 | 0.0 | 41.633373 | 42 |
GTATCTC | 148465 | 0.0 | 41.57969 | 38 |
TCGTATG | 148785 | 0.0 | 41.504528 | 43 |
GTATGCC | 148645 | 0.0 | 41.493984 | 45 |