FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l08n01 2-3h gd7 8wg16 rep2_150303.34100000007b86.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l08n01 2-3h gd7 8wg16 rep2_150303.34100000007b86.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48413980
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC13133102.7126668784512242TruSeq Adapter, Index 2 (100% over 51bp)
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1870750.38640698409839475No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1778990.36745378091204234No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA1548740.31989520382335845No Hit
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT1521850.31434102298550953No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1392250.2875718955557878No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1351360.2791259879894196No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1318520.27234282329195No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1233820.25484787658440805No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1216650.2513013803037883No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1166940.2410336848984529No Hit
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA540080.11155455510990833No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1469850.043.02542513
CACGTCT1470300.042.9373314
ACGTCTG1473350.042.81045515
CGTCTGA1474500.042.7709616
TCGGAAG1500450.042.6472173
CGGAAGA1504500.042.4696124
GATCGGA1520250.042.312321
CGATGTA1470500.042.2229334
CACACGT1501850.042.1521212
GTCACCG1479900.042.11009629
TATCTCG1468350.042.04454439
CCGATGT1480150.042.00237733
AGTCACC1486250.041.9298728
TCTGAAC1506800.041.7994418
ACCGATG1491850.041.7621232
TCTCGTA1475550.041.76179541
CTCGTAT1480750.041.63337342
GTATCTC1484650.041.5796938
TCGTATG1487850.041.50452843
GTATGCC1486450.041.49398445