FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l08n01 2-3h db 8wg16 rep2 input_150303.34100000007c1b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l08n01 2-3h db 8wg16 rep2 input_150303.34100000007c1b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48095396
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT2478650.5153611792696332No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA2233250.4643375844124456No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA2227550.4631524397886234No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1996170.4150438848658196No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA1993170.4144201245374921No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1741190.36202841536017294No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1543030.3208269664730487No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1512990.31458104638539625No Hit
CAACACAACACAACACAACACAACACAACACAACACAACACAACACAACAC1512430.3144646111241084No Hit
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA1486780.30913146031690847No Hit
AACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA1422580.2957829892907005No Hit
CAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGAC1422170.2956977420458291No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1371230.2851062916708285No Hit
CACAACACAACACAACACAACACAACACAACACAACACAACACAACACAAC1354250.281575808212495No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA1186170.2466285962174009No Hit
ACAACACAACACAACACAACACAACACAACACAACACAACACAACACAACA1161390.241476335905416No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1136600.2363219963923366No Hit
ACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGA1074260.22336025676969162No Hit
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACAA966690.20099429059696278No Hit
TGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT964290.20049528233430078No Hit
GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTG927470.1928396639046282No Hit
TGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT879020.18276593460213947No Hit
GACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAG846690.17604387746386368No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTG828200.17219943464027201No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACTTGAATCTCGTATGCC735490.15292316129385855TruSeq Adapter, Index 8 (100% over 51bp)
TTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGT665950.13846439688322768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTCTG143850.023.95746815
CGTCTGA143250.023.9478916
CACGTCT149050.023.16685514
ACACGTC149900.022.96045313
CTCGTAT148150.022.93182642
GTCACAC151200.022.31558629
AATCTCG156050.021.64103339
TCTCGTA159050.021.53001441
TCTGAAC161000.021.48966618
GTATGCC158750.021.46995245
TCGTATG160300.021.19303943
ATCTCGT164650.020.6884540
CAGTCAC164550.020.64134827
AGTCACA167350.020.25507228
GAATCTC171600.019.77152638
CGTATGC173750.019.59133144
GTCTGAA181450.019.1913717
CCAGTCA180750.019.17741426
CACACGT181850.019.00065612
TCCAGTC182050.018.97865125
Produced by FastQC (version 0.11.2)