Basic Statistics
Measure | Value |
---|---|
Filename | C55MVACXX l08n01 2-3h db 8wg16 rep2_150303.34100000007bd6.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 49798616 |
Sequences flagged as poor quality | 0 |
Sequence length | 51 |
%GC | 42 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC | 460465 | 0.9246542112736628 | TruSeq Adapter, Index 4 (100% over 51bp) |
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA | 144086 | 0.28933735829124246 | No Hit |
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA | 136612 | 0.27432890906044455 | No Hit |
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT | 129023 | 0.25908952971705074 | No Hit |
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC | 122452 | 0.2458943838921146 | No Hit |
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA | 117025 | 0.2349964906655237 | No Hit |
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT | 113843 | 0.2286067548543919 | No Hit |
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC | 109343 | 0.21957035914411757 | No Hit |
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT | 100452 | 0.20171644930855104 | No Hit |
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG | 97944 | 0.19668016476602482 | No Hit |
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG | 88869 | 0.17845676675030486 | No Hit |
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA | 51504 | 0.10342456103599346 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CACGTCT | 55290 | 0.0 | 40.169456 | 14 |
ACACGTC | 55395 | 0.0 | 40.158257 | 13 |
CGTCTGA | 55955 | 0.0 | 39.748547 | 16 |
ACGTCTG | 56020 | 0.0 | 39.69841 | 15 |
TCGGAAG | 57800 | 0.0 | 38.88818 | 3 |
TCTCGTA | 56445 | 0.0 | 38.524303 | 41 |
CACACGT | 58070 | 0.0 | 38.44007 | 12 |
CGGAAGA | 58700 | 0.0 | 38.207626 | 4 |
TCGTATG | 57025 | 0.0 | 38.206173 | 43 |
CTCGTAT | 57095 | 0.0 | 38.109367 | 42 |
ACTGACC | 57955 | 0.0 | 37.855183 | 32 |
GTATGCC | 57590 | 0.0 | 37.84977 | 45 |
ATCTCGT | 57700 | 0.0 | 37.76051 | 40 |
GTCACTG | 58445 | 0.0 | 37.74995 | 29 |
AATCTCG | 57785 | 0.0 | 37.69706 | 39 |
GATCGGA | 60125 | 0.0 | 37.58764 | 1 |
TCTGAAC | 59370 | 0.0 | 37.56119 | 18 |
CGTATGC | 58105 | 0.0 | 37.41472 | 44 |
CAGTCAC | 59115 | 0.0 | 37.2536 | 27 |
AGTCACT | 59640 | 0.0 | 36.980675 | 28 |