FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l08n01 2-3h db 8wg16 rep2_150303.34100000007bd6.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l08n01 2-3h db 8wg16 rep2_150303.34100000007bd6.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49798616
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC4604650.9246542112736628TruSeq Adapter, Index 4 (100% over 51bp)
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1440860.28933735829124246No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1366120.27432890906044455No Hit
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT1290230.25908952971705074No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1224520.2458943838921146No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA1170250.2349964906655237No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1138430.2286067548543919No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1093430.21957035914411757No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1004520.20171644930855104No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG979440.19668016476602482No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG888690.17845676675030486No Hit
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA515040.10342456103599346No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGTCT552900.040.16945614
ACACGTC553950.040.15825713
CGTCTGA559550.039.74854716
ACGTCTG560200.039.6984115
TCGGAAG578000.038.888183
TCTCGTA564450.038.52430341
CACACGT580700.038.4400712
CGGAAGA587000.038.2076264
TCGTATG570250.038.20617343
CTCGTAT570950.038.10936742
ACTGACC579550.037.85518332
GTATGCC575900.037.8497745
ATCTCGT577000.037.7605140
GTCACTG584450.037.7499529
AATCTCG577850.037.6970639
GATCGGA601250.037.587641
TCTGAAC593700.037.5611918
CGTATGC581050.037.4147244
CAGTCAC591150.037.253627
AGTCACT596400.036.98067528