FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l07n01 2-3h gd7 8wg16 rep1 input_150303.34100000007bc9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l07n01 2-3h gd7 8wg16 rep1 input_150303.34100000007bc9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55319655
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT3379050.6108226813778936No Hit
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA3020610.5460283510444885No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA3018350.5456198163202572No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA2770560.5008274183922513No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT2699900.4880543813948225No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG2287590.413522101683389No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC2222390.40173605565689086No Hit
AAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACA2180020.394076933415438No Hit
CAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGAC2056250.37170333039857173No Hit
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1986740.3591381761148004No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1937220.3501865656971288No Hit
AGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAA1754920.3172326363929782No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1559900.2819793435081979No Hit
CAACACAACACAACACAACACAACACAACACAACACAACACAACACAACAC1533500.2772070794729287No Hit
ACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGA1495700.2703740650587933No Hit
AACACAACACAACACAACACAACACAACACAACACAACACAACACAACACA1433950.25921166717326777No Hit
CACAACACAACACAACACAACACAACACAACACAACACAACACAACACAAC1391530.25154350655296026No Hit
GACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAGACAAG1207780.21832746426202407No Hit
ACAACACAACACAACACAACACAACACAACACAACACAACACAACACAACA1161680.20999407895801228No Hit
TGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGT1154170.20863651445403988No Hit
GTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTG1124810.20332917839057385No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC1086310.1963696266724729TruSeq Adapter, Index 5 (100% over 51bp)
TGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTT1058410.19132621127156343No Hit
GTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTG981550.1774324152961547No Hit
ACACAACACAACACAACACAACACAACACAACACAACACAACACAACACAA964290.17431236691552035No Hit
TTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGTTGTGT767960.13882226850474755No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGA188150.027.83293316
ACGTCTG191450.027.3766815
CACGTCT191650.027.26594714
ACACGTC193250.027.13332213
CTCGTAT194300.026.66503542
TCGTATG202700.025.52938343
AGTGATC203700.025.357936
TCTGAAC207500.025.29163218
GTGATCT204500.025.1706937
GTATGCC210400.024.64879645
GTCACAC210250.024.63529229
CGTATGC219850.023.56882744
TCTCGTA222050.023.45423541
CAGTCAC223900.023.35410327
ATCTCGT221100.023.3520640
AGTCACA222450.023.25228328
CAGTGAT224450.023.0655235
ACAGTGA226400.022.88672834
CACACGT231300.022.79618612
GTCTGAA233900.022.59087417
Produced by FastQC (version 0.11.2)