FastQCFastQC Report
Thu 9 Apr 2015
C55MVACXX l07n01 2-3h gd7 8wg16 rep1_150303.34100000007b93.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC55MVACXX l07n01 2-3h gd7 8wg16 rep1_150303.34100000007b93.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45630046
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGA1833850.4018952775107876No Hit
AGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGA1761140.3859606014861348No Hit
AGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAA1520670.3332606765287942No Hit
TTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCT1431180.3136485989954952No Hit
TCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCT1316780.2885773991987648No Hit
CTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTC1303100.28557937460768723No Hit
GAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAG1302290.28540185999374185No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC1291930.283131426165996TruSeq Adapter, Index 1 (100% over 51bp)
GAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAGAGAAG1179930.25858619559577034No Hit
CTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTC1157450.25365961717417507No Hit
TCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTTCTCTT1097070.24042710805069095No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC185700.032.36705413
CGTCTGA187700.032.0101916
CACGTCT189900.031.69857814
ACGTCTG190050.031.69723315
TCACGAT189750.031.1728835
TCGTATG195900.030.48094443
TCGGAAG202950.029.915243
CATCACG198950.029.90096333
TCTCGTA199700.029.79646941
CACGATC200900.029.30594636
CTCGTAT205250.029.07845142
ATCACGA206400.028.89802734
CGTATGC207000.028.87944844
ACGATCT207050.028.59836237
CACACGT213950.028.46129212
GTATGCC212100.028.26989645
ATCTCGT212550.028.03815840
CGGAAGA217450.027.9411224
GATCGGA228050.026.7872561
TCTGAAC226600.026.71360218
Produced by FastQC (version 0.11.2)